Journal: Frontiers in Plant Science

Article Title: Assessing seed characteristics for improved winter survival of late-fall-seeded lentils

doi: 10.3389/fpls.2026.1802566

Figure Lengend Snippet: Steps involved in the LT 50 freezing test. Seeds were nucleated with shaved ice at -2 °C and the temperature was subsequently lowered at the rate of 2 °C for one hour. Samples were removed at various temperatures, thawed overnight at 2 °C, and then placed into a germination chamber at 23 °C in the dark.

Article Snippet: Thawed seeds were then germinated in Petri dishes lined with hydrated triple Whatman #1 filter paper, without free-standing water, at a constant temperature of +23 °C in darkness in the germination chamber (Powers Scientific, Inc., DS33SD, Ontario) ( ).

Techniques:

Journal: Methods and Protocols

Article Title: Development of an Application Method for Volatile Compounds Derived from Mushroom Fungi Beds as Plant Growth-Promoting Biostimulants

doi: 10.3390/mps8020029

Figure Lengend Snippet: An overview of the schematic workflow of non-contact VCs exposure on rice seedlings experimental design and conditions. ( 1 ) Rice seeds were sterilized with 1% sodium hypochlorite. After 4 days of germination, rice seedlings were transplanted into a plant box with culture soil. Seedlings are exposed to mushroom fungi bed-derived VCs (+VCs) in a non-contact setup, while control seedlings remain unexposed (−VCs). Growth conditions include a 13 h light/11 h dark photoperiod, 123 ± 6 μmol m⁻ 2 s⁻ 1 and 122 ± 9 μmol m⁻ 2 s⁻ 1 PPFD, and around 60% relative humidity for 14 days. ( 2 ) HS-SPME-GC-TOF-MS analysis identified 3-octanone, 1-octen-3-ol, 1-octen-3-one, and 3-octanol as key fungal VCs present in shiitake mushroom FBs. Illustration was created using Biorender; https://BioRender.com (accessed on 3 March 2025).

Article Snippet: The list of equipment used during this study is as follows: Growth chamber for germination (PHCbi MLR-352, PHC Corp., Tokyo, Japan); Growth chamber for VCs exposure test (FLI-2000A; TOKYO RIKAKIKAI Co., Ltd., Tokyo, Japan); Alcohol lamp (Maruemu Corp., Osaka, Japan); Paper towel, Kim Towel (Nippon Paper Crecia Co., Ltd., Tokyo, Japan); Sterile disposable plastic Petri dishes (φ90 × 15 mm, Az One Corp., Tokyo, Japan); Autoclave (HICLAVE HVE-50, HIRAYAMA Manufacturing Corp., Tokyo, Japan); GA-7 Magenta boxes (77 × 77 × 97 mm; Merck KgaA, Darmstadt, Germany); LI-250A Light meter (LI-COR Environmental, Lincoln, NE, USA); Filter paper (MerkMillipore, Merck KgaA, Burlington, MA, USA, CAT No. RAWP02500); Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) fiber assemblies of 65 μm (Pink/plain) (Supelco ® , Merck KgaA, Darmstadt, Germany); Jeol JMS-T100GCV type GC-TOF Mass Spectrometer (JEOL Co., Ltd., Datum Solution Division MS Service Department, Tokyo Japan); Agilent 7890A GC System with FID, serial number CN14333069 (Agilent Technologies, Santa Clara, CA, USA); Plastic containers of 3 L (Sanada Seiko Co., Ltd., Osaka, Japan); High-density polyethylene (HDPE) film of 10 μm thickness (Fukusuke Kogyo Co., Ltd., Ehime, Japan); Polyvinyl chloride (PVC) film of 9 μm thickness (Pack Style, Co., Ltd., Aichi, Japan); A 18-8 Extra Thick Measuring Spoon 1/4 Spoon (Wadasuke Co., Ltd., Niigata, Japan); Clear glass vial (Supelco ® ) of 15 mL with a screw cap fitted with a PTFE/silicone septum (Merck KgaA, Darmstadt, Germany).

Techniques: Derivative Assay, Control